Hek293 and HeLa cells are widely used in research for their adaptability and ease of growth. Proper lab setup, sterile techniques, and careful media selection are essential for optimal cell maintenance. Regular subculturing and monitoring ensure healthy growth, while transfection optimization enhances experimental outcomes. Advanced methods like CRISPR/Cas9 and 3D cultures further expand the capabilities of these cells, allowing for breakthroughs in areas like gene editing, cancer research, and drug development, making them invaluable tools in biomedical science.

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