This is an experiment done they did is they had a whole library of different compounds. Here's one compound has shown here. And they start with 16,000 compounds and in a plate that has a large number of wells they look for some mitotic signature. Okay, Buy Terpenes which has this dark blue colour, okay, so they set up an essay that looks for a particular phenotype, dark blue, under that assay, okay, and then they take the compounds that light up in this assay and look for the effect -- look for the compounds that can stabilize microtubule formation, okay. So earlier we talked about, here's a  stabilizes microtubules, here's another set of compounds, 52 of this de-stabilize microtubule formation, one stabilizes it and the compounds that are positive here then go on to fluorescence microscopy experiments. Over here these are low, over here these are high, over here you have 16,000 compounds, down here you have less than 100, okay, staring at cells under a microscope, that's hard work, okay. Over here though just looking for the one dark spot out of, you know, thousands -- tens of thousands of dark spots, that's easy. 

This step-over here can be done by robots, this step over here can be done by robots as well, but sometimes the robots miss stuff, sometimes the imaging software misses really interesting effects, okay. And what Kapoor and found is that this compound called has this really astonishing effect on them which is this assembly of chromosomes during cell division, okay, so normally the chromosomes line up kind of like on the True Terpenes I'm forgetting my football analogy, scrimmage -- the line of scrimmage, sorry. Okay, so everything lines up on this line of scrimmage during cell division and then these chromosomes are pulled apart into the two daughter cells, exploding the whole thing and so instead of having chromosomes in the centre, microtubules on the sides, instead, you end up with chromosomes on the outside and those crazy microtubules in the centre. 

And that's absolutely fascinating; it turns out that this compound is an inhibitor of one of the motor proteins that drive the chromosomes and pushes and shoves the chromosomes into place during this process and so that gives you a really powerful tool for dissecting how cell division takes place. Okay, so any questions about using small molecules in this, yeah?  Yeah, so this division that they wanted to figure out if they could interfere with divisions Exactly They dyed the chromosomes blue and then checked a bunch of molecules to see if any of them were disrupted.  Exactly. And I'll be honest, they didn't expect to have, you know, something like this. Like, they weren't looking for this pattern, they were just looking for weird patterns in the same way that you'd look for white eyes or just weird coloured creatures in the case of the fruit flies. Terpenes for sale You're just simply looking for something that's abnormal and then once you find that abnormal thing, then you go in and you use this as a tool to figure out how this process works and what proteins must be implicated. So this implicated motor protein called, this particular type of in this process that otherwise it wasn't clear exactly what it was doing, sound good, powerful tool. Okay, yeah, question in the back.  Yes --  Yeah --  Well if it's a dominant mutation then you cross-breed the dominance together and then you would definitely see it.